ASSESSMENT OF THE INFLUENCE OF DIET USED FOR INFANTS AND SMALL CHILDREN ON THE NEUROPRESCOSITION SYSTEM – NEW EIT + PROJECT
What effect does the diet have on the neurotransmitter system of infants and young children? The answer to this question is sought by dr Anna Czajkowska-Mysłek from the Wrocław Research Centre EIT +, who launched a research project on the Application of molecular sorbents with molecular imprint (MIP-SPE) for selective profiling of catecholamines and their metabolites. The research received funding from the National Science Centre as part of the MINIATURA 1 program.
The project BabyMIP (project acronym) aims to broaden the knowledge on the use of innovative polymer sorbents with molecular imprinting (MIP-SPE) for profiling catecholamines and their metabolites in a minimal amount of body fluid. Catecholamines are organic chemical compounds derived from the tyrosine – amino acid. Catecholamines are soluble in water and circulate in the blood in 50% combined with plasma proteins. When the concentration is the highest, we are already talking about adrenaline, noradrenaline and dopamine, because the high level of catecholamines in the blood is primarily associated with stress. Catecholamines evoke general body reactions that prepare the body for physical exercise related to fighting or escaping. Typical effects are: elevation of blood pressure, acceleration of the heart rate, as well as raising the level of glucose in the blood. What can cause stress? Stress can be triggered as a psychological reaction, it can arise in response to environmental stressors, such as noise or intense light, but high levels of catecholamines can also result from diet.
As part of the project, which will be carried out in the Mas Spectrometry Laboratory at the Wrocław Research Centre EIT +, a new method of catecholamine analysis using the MIP-SPE extraction will be developed. The research will concern body fluids in which the analysed biomarkers are present at a very low concentration level. The currently used methods of sample preparation are not sufficiently selective in relation to such a complex matrix as are samples of biological origin (blood, urine). In addition, conducting research using samples from small children, especially infants, involves obtaining the minimum amount of material collected for testing.
– The use of MIP-SPE will allow selective separation of compounds that are structural and functional analogs of catecholamines, as well as their concentration. Extraction of MIP-SPE combined with ultra-high-performance liquid chromatography and mass spectrometry techniques will enable testing in a small amount of material, with sensitivity sufficient to evaluate the transformation of these compounds under the influence of diet – explains Dr. Anna Czajkowska-Mysłek. – This will allow obtaining much lower limits of detection of analytes, simplification of the sample preparation protocol and significantly lower consumption of organic solvents used in the analysis.
Thanks to the innovative extraction technique combined with very sensitive analytical techniques, there is a chance that we will be able to determine the transformation of catecholamines, which in turn will contribute to the continuation of research conducted by Dr Czajkowska-Mysłek on the influence of the diet used in infants and young children on the neurotransmitter system.
Posted by Wrocławskie Centrum Badań EIT+, Posted on 05.02.2018