Multiparameter characterization of surveillance of reproductive epithelium homeostasis by gamma-delta T cells that includes high resolution intravital microscopy.
Grzegorz Chodaczek PhD
01.07.2015 r. – 30.06.2020
1 340 184,00 PLN
AIM OF THE PROJECT
Among many cell types engaged in the process of mucosal surveillance γδ T cells play a unique role. The prevailing view in the mucosal immunology is that γδ T lymphocytes maintain tissue homeostasis by recognizing self-antigens which are expressed upon wounding or infection. In contrast, our published research, which utilized a novel technique being a combination of intravital confocal microscopy with immunofluorescence (Chodaczek et al. Nature Immunology 2012), shows that skin γδ T lymphocytes are in a permanent state of heightened activation through constitutive γδ TCR phosphorylation, which immobilizes them in epidermis suggesting continuous presence of a cognate ligand in healthy tissue. It is not known whether similar mechanisms apply to other epithelia hosting similar γδ T cell populations. The project aims at filling the gap in knowledge through 1) analysis of activation status of intraepithelial T cells, 2) analysis of epithelial functions and induction of immune responses modulated by intraepithelial T cells, 3) visualization of epithelial leukocyte dynamics in real time including cell motility and cellular interactions.
Posted by Agata Kołacz, Posted on 20.06.2016