PROTEIN BIOCHEMISTRY

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PROTEIN BIOCHEMISTRY

PROTEIN PRODUCTION AND PURIFICATION FORM VARIOUS EXPRESSION SYSTEMS

  • Construction of expression vectors:
    – expression of native proteins
    – expression of tagged proteins
  • Optimization of target gene expression conditions:
    – expression systems selection
    – identification of clones with high expression of target protein
    – selection expression conditions (temperature, expression time, medium composition, inducer concentration)
  • Adaptation to production in different types of bioreactors:
    – fed-batch system
    – perfusion system

See also: Cell Culture Laboratory

QUALITATIVE AND QUANTITATIVE PROTEIN ANALYSIS

  • identity(MS, MS/MS)
  • purity (PAGE, Western Blot, SEC, UV-Vis, HPLC/FPLC)
  • activity (SPR, ELISA, AlphaScreen, AlphaLISA, UV-NIR, FRET, TR-FRET, TRF, TP)
  • aggreation/oligomerization (DLS, SEC)
  • stability (DSC, CD)
  • structural analysis (CD, FTIR)
  • amino acid analysis (MS)
  • protein sequencing (MS)
  • proteomics (MS)
  • proteomics and phosphoproteomics (based on radioisotopic methods)

CUSTOMER SPECIFIED PROTEIN DESIGN AND MODIFICATIONS

MOLECULAR INTERACIONS ANALYSIS

  • kinetics and biophysics of protein-protein, protein-ligand & DNA interactions
  • mechanism (Kd, DH, DS, stechiometry) and kinetics (Kd, kon, koff) of interactions for interactants ranging from ions to viruses
  • enzyme kinetics parameterization (kcat, Vmax, Km, IC50, Ki)
  • biological activity analysis (SPR, BLI, MST, ELISA, AlphaScreen, AlphaLISA, UV-NIR, FRET, TR-FRET, TRF, FP)
  • potential drug targets and diagnostic markers development

See also: Protein Engineering Laboratory

Posted by Agata Kołacz, Posted on 27.10.2016
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