MICROSCOPIC ANALYSIS

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MICROSCOPIC ANALYSIS

SAMPLE PREPARATION

  • In-situ preparation of ultrathin sections (thickness below 30 nm) for TEM observations using focused ion beam and micromanipulator
  • High pressure freezing and freeze-substitution
  • Blocks slicing using ultramicrotome
  • Critical point drying for scanning electron microscopic study

See also: Electron Microscopy Laboratory

SEM ANAYSIS

  • Observations of biological specimens using field emission SEM

– max. resolution ~0,8 nm

  • Observations of biological specimens using transmission electron microscopy STEM

– magnifications up to 2 million times

– up to 12 specimens for series

  • Observations of native biological material using highly sensitive, energy-selective backscattered electron detector ESB® at normal and cryo conditions

– nano-scaled clear compositional contrast

– work below 1,5 kV allowing for selecting the desired energy of backscattered electrons

  • Analysis of biological specimens by serial imaging of the surfaces revealed by gallium ion milling

– collection of serial images desired for three-dimensional reconstruction of subcellular features

  • Analysis of elemental composition and distribution within the biological specimens using energy-dispersive X-ray spectrometer

See also: Electron Microscopy Laboratory

 WIDEFIELD AND CONFOCAL MICROSCOPY ANAYSIS

  • Qualitative and quantitative analysis of microscopic images (cultured cells and tissue sections; thickness to 0,2 mm):
  • cell morphology and distribution
  • intracellular organelles distribution
  • protein colocalization
  • cytoskeleton
  • In-vitro live microscopic analysis of cultured cells and tissues (controlled environment: temperature, humidity, CO2 and O2 ):
  • cell morphology and motility
  • migration studies on cells and intracellular structures
  • functional cell assays (free radical production, phagocytosis, cytoskeletal changes, antibody internalization)
  • In-vivo microscopic analysis of cell distribution and motility in a mouse model in real time
  • 3D visualistation
  • High throughput screening
  • FRAP experiments (fluorescence recovery after photobleaching)

See also: Electron Microscopy Laboratory

Posted by Agata Kołacz, Posted on 27.10.2016
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