Cell Culture Laboratory

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Cell Culture Laboratory

Cell and tissue culture has become one of the major tools used in life sciences today. Cell line based in vitro studies are alternatives to animal testing.
Cell Culture Laboratory is involved in the area of cell line development, selection and optimizing cell culture technology. Responding to current bioprocessing challenges we integrate modern cell biology research and
tissue and microbes culture innovative technologies.

We use advances in cell and molecular biology including genetic engineering, omics technologies and synthetic biology.

Facility equipment meets the highest standards necessary to grow animal
cells and microbes in controlled conditions and produce biologics using
recombinant DNA technology.

  • TUMOR CELL LINE GENERARTION
    Generation of immortal tumor cell lines from fresh tumors
  • PRODUCTION OF RECOMBINANT PROTEINS:
    Expression systems:
    – prokaryotic systems (bacterial E. Coli)
    – eukaryotic systems:
    – yeast (Pichia pastoris, Saccharomyces cerevisiae)
    – insect cells
    – mammalian cells (CHO, Hek293)
  • CONSTRUCTION OF EXPRESSION VECTORS:
    – expression of native proteins
    – expression of tagged proteins
  • OPTIMIZATION TARGET GENE EXPRESSION CONDITIONS:
    – expression systems selection
    – identification of clones with high expression of target protein
    – selection expression conditions:
    – temperature
    – expression time
    – medium composition
    – inducer concentration
    – adaptation to production in different types of bioreactors:
    – fed-batch system
    – perfusion system
  • CELL LINE DEVELOPMENT:
    – design and construction of expression vector
    – selection of transfection method
    – selection gene amplification system (GS, DHFR)
    – isolation stable clones with high expression
  • Class II Laminar Flow Cabinets – LaboGene
  • CO2 incubators – Binder
  • Bioreactors: Biostat STR 50l, Biostat RM, Biostat B Twin – Sartorius
  • Roller bottles incubator
  • Reverse-phase contrast microscopes
  • Electroporators
  • Automatic cell counter
  • Dewar
  • Microbial incubators
  • Microbial bioreactors

Sales  Department
Tel: +48 71 720 16 45
e-mail: laboratoria@eitplus.pl

Posted by Agata Kołacz, Posted on 10.02.2016
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