Biospectroscopy and Bioimaging Laboratory

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Biospectroscopy and Bioimaging Laboratory

The Laboratory of Biospectroscopy and Bioimaging (LBB) contains advanced measurement instruments along with auxiliary optical, spectroscopic and photodetector components, which enables undertaking innovative and original research in a wide area of biospectroscopy and bioimaging.

In particular, the laboratory allows for:

  • designing new, unique, and personalised experiments in biospectroscopy and bioimaging domain based on a fully automated optical fluorescence microscope, programmable array microscope and other advanced laboratory equipment
  • designing, synthesis and bio-functionalization of lanthanide-doped luminescent nanoparticle labels, as well as their versatile spectroscopic characterisation, further optimisation and application in vitro

We are focused on luminescent nanoparticles’ chemistry and physics, light, spectroscopy and design of optical instrumentation to design novel luminescent materials and (bio)detection methods or instruments.

THE SERVICES

  • Cooperation with partners from academia and industry within fundamental as well as applied research projects
  • Designing, automation and testing specialized biodetection methods and building prototype instruments
  • Expert consultations in the areas of biospectroscopy, biodetection and bioimaging
  • Training courses in biospectroscopy, laser safety and applications of nanoluminophores
Artur Bednarkiewicz Artur Bednarkiewicz, dr hab. eng.

  • tel. +48 71 734 7216
  • e-mail: artur.bednarkiewicz (at) eitplus.pl
  • building 4, room 4.41

Artur Bednarkiewicz received his MSc Eng. in Physics and Biomedical Engineering from the Wroclaw University of Technology in Wroclaw, Poland, in 1993 and a PhD and habilitation in Physics from the Institute of Low Temperature and Structure Research, Polish Academy of Sciences, in 2003 and 2013 respectively. After 4 year lasting postdoctoral research in the European Commission Joint Research Centre, Italy, he returned to ILT&SR PAS in December 2008 and simultaneously established an interdisciplinary team NAOMIS with the Wroclaw Research Centre EIT+ focusing on Nanoparticle Assisted Molecular Imaging and Sensing (NAOMIS). His current interdisciplinary research focuses on optical (bio)spectroscopy, novel designs of lanthanide doped nanoparticles and quantum dot biolabels, optical and spatial light modulation engineering, as well as on pure physics, spectroscopy and chemistry of lanthanide doped materials. Since 2015 he is an associated professor at Institute of Low Temperature and Structure Research, PAS, Poland. He is a co-author of over 90 publications (h-index >18, cited>1050, sum IF>150), he is an author of 4 patents and 3 patents applications. In the course of his research he has received numerous prizes (stipends from Ministry of Education, FNP Starting grant, Brussels Eureca). He has a broad experience as researcher (3 research grants as PI), reviewer (over 60 reviews of scientific papers, over 30 reviews of national (FNP,NCN) and international (EU FET Open RIA, Australian, Belgian) grants, reviewers of 2 Ph.D. theses, scientific supervisor to 6 M.Sc. and 2 Ph.D. students, entrepreneur (co-owner of Nanovectors Ltd. Company). Since 2014, he is also a member of Young Academy of Science, where he is engaged in widening European participation of new EU member countries. He is also Polish representative and active participant and collaborator of COST CM1403 European Upconversion Network (STSM manager, Web designer).

 

Małgorzata Misiak, MS

  • tel.+48 71 734 7218
  • e-mail:Malgorzata.Misiak (at) eitplus.pl
  • position : researcher

Katarzyna Prorok, MS

  • tel.+48 71 734 7218
  • e-mail:Katarzyna.Prorok (at) eitplus.pl
  • position : researcher

Agnieszka Kowalczyk, MS

  • tel.+48 71 734 7218
  • e-mail: Agnieszka.Kowalczyk (at) eitplus.pl
  • position : researcher
  • Fully automated inverted fluorescence microscope CarlZeiss AxioObserver equipped with:
    – dry objectives 10 x, 20 x, 40 x
    – immersion objectives 40x, 63x (NA=1.43)
    – ultrasensitive 1-Megapixel cameras (EMCCD Rolera EMc2) in dual-camera configuration that enable simultaneous image recording in the same area of the sample in 2 spectral ranges
    – standard 5 Mpix colour camera (Zeiss MRc5)
    – capacity to record fluorescence spectra from under the microscope with ultrasensitive spectrograph
    – capacity to work in white light, phase contrast, and Nomarski interference contrast imaging modes
    – full automation, with the option to integrate the automated microscope into custom control and measurement applications (LabView)
    – CO2 incubator + temperature control for long-term in vitro measurements
    – capability to carry out imaging in up-conversion mode (980 nm excitation, emission in Vis range) or NIR mode (808 nm excitation, 840-1050 nm emission)
    – capability to provide dynamic, spatially controlled initiation of photochemical reactions (e.g. photosensitizers’ activation, photolithography, etc.) using DMD technology (Digital Micro-mirror Device, Texas Instruments) integrated with the microscope. Available LED source of 375 nm wavelength and 1024×768 array allows for optical resolutions of around 10 μm
  • Extensive selection of opto-mechanical components that enables building custom optical and measurement setups
  • A set of optical-fiber semiconductor and “free-running” CW, quasi-CW and pulsed lasers (including 980 nm CW 3W, 808 nm CW 2W, and picosecond lasers up to 40 MHz at 375 and 405 nm wavelength)
  • Tunable Ti-sapphire laser in 700 – 1000 nm and 350 – 500 nm spectral range, under 532 nm pump (>100 mJ in 10 ns pulse)
    Photomultiplier tubes (400 – 1000 nm) as photodetectors and photon counters
  • Power and energy meters, digital oscilloscopes, oscilloscope cards, function generator
  • Shemrock SR303i (Andor) spectrograph with ultrasensitive EM-CCD detector (Newton 1600×400 back-thinned) and a photomultiplier
    tube, as well as 3 gratings and customized light coupling system
    Miniature CCD spectrophotometers (OceanOptics), including HR4000 + and cooled QE65000
  • A set of sources, detectors and devices for measuring ultrashort fluorescence lifetime (pico-nanoseconds) using the Time Correlated
    Single Photon Counting (TCSPC) technique Capability to develop and automate custom control applications (LabView + NI Vision) and optical/spectroscopic measurement systems
  • Fully equipped chemical and spectroscopy laboratory, which are ready for the synthesis and spectroscopic characterization of nanocolloidal luminophores (lanthanide doped NaYF4, CaF2, core-shell structures, CuInS2 /ZnS/CdS etc. quantum dots, colloidal gold/silver nano-particles)

CURRENTLY RUNNING PROJECTS:

 „Synteza i optymalizacja właściwości spektroskopowych koloidalnych nanokrystalicznych tlenków domieszkowanych jonami lantanowców”. National Centre of Science (NCN, Poland), Preludium project, grant no. [09.2014 – 09.2016] – K.Prorok

National Centre of Science (NCN, Poland), Preludium project, grant no. DEC-2013/11/N/ST5/02716. [09.2014 – 09.2016] – M.Misiak

FINISHED PROJECTS:

Nanoparticle Assisted Molecular Imaging and Sensing (NAOMIS)  1.2012-12.2015 – “The Application of Nanotechnology in Advanced Materials” – NanoMat (POIG.01.01.02-02-002/08) financed by the European Regional Development Fund (Operational Programme Innovative Economy 1.1.2).

2016

  1. Chapter 9: (Active-Core-Active-Shell Upconverting Nanoparticles: novel mechanisms, features and perspectives for bio-labeling) by Katarzyna Prorok, Dominika Wawrzyńczyk, Małgorzata Misiak, Artur Bednarkiewicz to the book Upconverting Nanomaterials: Perspectives, Synthesis, and Applications, CRC Press, Claudia Altavilla
  2. Bazylińska, D. Wawrzyńczyk, J. Kulbacka, R. Frackowiak, B. Cichy, A. Bednarkiewicz, M. Samoć, K. Wilk, Polymeric nanocapsules with up-converting nanocrystals cargo make ideal fluorescent bioprobesScientific Reports 6, Article number: 29746 (2016), doi:10.1038/srep29746
  3. Revisiting the classification of NIR absorbing/emitting Nanomaterials for in vivo bio-applications Joao Conde, Tzu-Ming Liu, Tomasz Lipiński, Artur Bednarkiewicz, and Chih-Chia Huang NPG Asia Materials (2016) 8, e295; doi:10.1038/am.2016.106
  4. Energy migration up-conversion of Tb3+ in Yb3+ and Nd3+ codoped active-core / active-shell NaYF4 colloidal nanoparticles Katarzyna Prorok, Mirosława Pawlyta, Wiesław Stręk, Artur Bednarkiewicz*, Mater. 2016, 28, 2295−2300
  5. Broadening temperature sensitivity range with core-shell YbEr@YbNd double ratiometric optical nanothermometer, L. Marciniak, K. Prorok, L. Francés-Soriano, J. Pérez-Prieto and A. Bednarkiewicz*, Nanoscale 2016, DOI: 1039/C5NR08223D
  6. Cytotoxic interactions of bare and coated NaGdF4:Yb3+:Er3+ nanoparticles with macrophage and fibroblast cells, E. Wysokińska, J. Cichos, E. Zioło, A. Bednarkiewicz, L. Strzadala, M. Karbowiak, D. Hreniak, W. Kałas, Toxicology in Vitro 32(2016) 16-25.
  7. Modulation of Thulium Upconversion in Potassium Tetraphosphates (KLaP4O12) nanocrystals by co-doping with Yb3+ ions Marciniak, A.Bednarkiewicz, M.Stefanski, R.Tomala, D.Hreniak and W.Strek, Journal of Materials Chemistry C, 2016, DOI: 10.1039/C5TC03102H

2015

  1. Neodymium-doped nanoparticles for infrared fluorescence bioimaging: the role of the host del Rosal, A. Pérez-Delgado, M.Misiak, A.Bednarkiewicz, A.S. Vanetsev, Yu. Orlovskii, D. J. Jovanović, M. D. Dramićanin, U. Rocha, K. Upendra Kumar, C. Jacinto, E. Navarro, E. Martín Rodríguez, I. R. Martín,  A. Speghini, M. Pedroni, G. A. Hirata, and D. Jaque, J. Appl. Phys. 118, 143104 (2015)
  2. Upconverting nanoparticles: assessing the toxicity, A.Gnach,   T.Lipinski,   A.Bednarkiewicz, J.Rybka and   J.A. Capobianco, Chem Soc Rev 2015 Mar;44(6):1561-84, DOI: 10.1039/C4CS00177J

 2014

  1. The impact of shell host (NaYF4/CaF2) and shell deposition methods on the up-conversion enhancement in Tb3+, Yb3+ codoped colloidal α-NaYF4 core–shell nanoparticles Katarzyna Prorok, Artur Bednarkiewicz, Bartlomiej Cichy, Anna Gnach, Malgorzata Misiak, Marcin Sobczyk and Wieslaw Strek Nanoscale, 2014, 6, 1855-1864 DOI: 10.1039/C3NR05412H

 2013

  1.  Influence of Li+ doping on up-conversion and structural properties of Yb3+/Tm3+-doped cubic NaYF4 Małgorzata Misiak, Bartłomiej Cichy, Artur Bednarkiewicz, Wiesław Stręk, Journal of Luminescence, Volume 145, January 2014, Pages 956-962
  2. The study of time-resolved collective emission of CuInS2 quantum dots in colloidal solutions, B.Cichy, A.Bdnakliewicz, W.Strek Journal of Optics 15 (2013) 085303 (5pp)
  3. Energy up-conversion in Tb3+/Yb3+ co-doped colloidal α-NaYF4 nanocrystals. Prorok K, Gnach A, Bednarkiewicz A, Stręk W. Journal of Luminescence. doi:10.1016/j.jlumin.2013.03.012.
  4. Third-order nonlinear optical response of CuInS2 quantum dots—Bright probes for near-infrared biodetection, B. Cichy, D. Wawrzynczyk, A. Bednarkiewicz, M. Samoc, and W. Strek Appl. Phys. Lett. 102, 243702 (2013);
  5. Up-converting NaYF4: 0.1Tm3+, 20%Yb3+ nanoparticles as luminescent labels for deep-tissue optical imaging, A. Gnach, K. Prorok, M. Misiak, B. Cichy, A. Bednarkiewicz – Journal Of Rare Earths, Vol. 32, No. 3, Mar. 2014, P. 207
  6. Thulium concentration quenching in the up-converting <alpha>-Tm3+/Yb3+ NaYF4 colloidal nanocrystals M.Misiak, K.Prorok, B.Cichy, A.Bednarkiewicz, W.Strek, Optical Materials 35(5), 1124–1128 (2013)

2012

  1. Lanthanide doped Up-converting Nanoparticles: merits and challenges, Anna Gnach, and Artur Bednarkiewicz, NanoToday 7(6), 532–563 (2012)
  2. Biological applications of lanthanide doped nanomarkers, (review) Małgorzata Misiak, Katarzyna Prorok, Artur Bednarkiewicz, Wiadomości Chemiczne (PL), 66(5-6), 1-49 (2012)
  1. „Energy up-conversion in Tb3+/Yb3+ co-doped colloidal α-NaYF4 nanocrystals”
    Prorok, A. Gnach, A. Bednarkiewicz; 8th International Conference on f-Elements (ICFE 2012), 26-31.08.2012, Udine, Włochy – plakat
  2. „Thulium concentration quenching in the up-converting colloidal Tm3+/Yb3+   α-NaYF4  nanocrystals”, Misiak, K. Prorok, A. Bednarkiewicz, 8th International Conference on f-Elements (ICFE 2012), 26-31.08.2012, Udine, Włochy  – plakat
  3. „Up-conversion enhancement by surface passivation of Tb3+, Yb3+ co-doped colloidal
    α-NaYF4 nanoparticles”; K. Prorok, A. Gnach, B. Cichy, A. Bednarkiewicz; International Conference of Rare Earth Materials Advances in Synthesis, Studies and Applications (REMAT), 26-28.04.2013, Wrocław, Polska – plakat
  4. „Up-converting NaYF4:0.1Tm3+,20%Yb3+ nanoparticles as tool for high resolution deep-tissue optical imaging”; A. Gnach, A. Bednarkiewicz, K. Prorok, M. Misiak, B. Cichy; International Conference of Rare Earth Materials Advances in Synthesis, Studies and Applications (REMAT), 26-28.04.2013, Wrocław, Polska – plakat
  5. „Up-conversion enhancement by surface passivation of Tb3+, Yb3+ co-doped colloidal
    α-NaYF4 nanoparticles”; K. Prorok, A. Gnach, B. Cichy, A. Bednarkiewicz; The Fourth International Workshop on Advanced Spectroscopy and Optical Materials (IWASOM 2013), 14-19.08.2013, Gdańsk, Polska – plakat
  6. „Energy migration upconversion in active-core/active-shell NaYbF4:Tb3+@NaYbF4:Nd3+ colloidal nanoparticles”; K. Prorok, A.Bednarkiewicz; International Conference of Fluorescent Up-converting Nanoparticles: a Platform for Energy and Biomedical Applications (FUN-Bioenergy), 4-6.06.2014, Torremolinos, Hiszpania, – plakat
  7. „Energy migration upconversion in active-core/active-shell NaYbF4:Tb3+@NaYbF4:Nd3+  colloidal nanoparticles”; K. Prorok, A.Bednarkiewicz; The International Conference on Luminescence, 13-18.07.2014, Wrocław, Polska – plakat
  8. “Cooperative Energy Upconversion Enhancement in Tb3+/Yb3+ β-NaYF4nanocrystals through Mn2+ co-doping”, Prorok, A.Bednarkiewicz, The Fifth International Workshop on Advanced Spectroscopy and Optical Materials (IWASOM 2015), 19- 24.07.2015, Gdańsk, Polska – plakat
  9. “Up- and down-conversion luminescence of Tb3+/Yb3+ codoped lanthanide oxide nanoparticles”, Prorok, A. Pilch, A.Bednarkiewicz, 1st Conference and Spring School on Properties, Design and Applications of Upconverting Nanomaterials, 23-27.05.2016, Wrocław, Polska – wykład i plakat
  10. “Green dual-mode luminescence from Tb3+/Yb3+  codoped Y2O3 nanoparticles” K. Prorok, A. Pilch, A.Bednarkiewicz, Rare Earths 2016, 5-10.06.2016, Sapporo, Japonia. – plakat

Contact:

Sales Department
Tel: +48 71 720 16 45
e-mail: laboratoria@eitplus.pl

Posted by Agata Kołacz, Posted on 08.10.2015
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